Transgene-free, virus-based gene silencing in plants by artificial microRNAs derived from minimal precursors.
Cisneros AE, Martin-Garcia T, Primc A, Kuziuta W, Sanchez-Vicente J, Aragones V, Daros JA, Carbonell A
Nucleic Acids Res. 2023 Oct 27;51(19):10719-10736. doi: 10.1093/nar/gkad747.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 199559 | pENTR-BS-AtMIR390a-B/c | GATEWAY-compatible entry vector |
| 199560 | pMDC32B-BS-AtMIR390a-B/c | Plant expression vector (2x35S) for direct cloning of amiRNAs into Arabidopsis thaliana MIR390a precursor including only the basal stem |