Two-Color CRISPR Imaging Reveals Dynamics of Herpes Simplex Virus 1 Replication Compartments and Virus-Host Interactions.

Xu H, Wang J, Deng Y, Hou F, Fu Y, Chen S, Zou W, Pan D, Chen B
J Virol. 2022 Dec 21;96(24):e0092022. doi: 10.1128/jvi.00920-22. Epub 2022 Dec 1. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
199446	pCA15-Ubc-NLS- scFV-tdTomato	Expression of scFV-tdTomato that binds to the GCN4 peptide from the SunTag System in mammalian cells
199447	pCA16-Ubc-NLS-HA-dSaCas9-NLS-[10XGCN4]-P2A-BSD	Expession of dSaCas9-10XGCN4 that recruits scFV-tdTomato (pCA15) for amplifying fluorescent signal
199448	pCA17-CRISPR-Tag (11XTS1) for dSpCas9	CRISPR-Tag sequence that can be efficiently bound by dSpCas9