A simple, rapid, and efficient method for generating multigene-knockout culture cells by the CRISPR/Cas9 system.
Fujii T, Inoue N, Nobeyama T, Inoue J, Ogasawara S, Otani Y, Fujii S, Ito C, Fukunaga R
Genes Cells. 2023 Mar 2. doi: 10.1111/gtc.13021.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 199331 | pUREF-EX | A pEF-BOS-EX-derived expression vector that contains an SV40 promoter-driven puromycin resistance gene |