Local changes in microtubule network mobility instruct neuronal polarization and axon specification.
Burute M, Jansen KI, Mihajlovic M, Vermonden T, Kapitein LC
Sci Adv. 2022 Nov 4;8(44):eabo2343. doi: 10.1126/sciadv.abo2343. Epub 2022 Nov 4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 193713 | pB80-2xFKBP-L-mCherry-CAAX | Rapalog-inducible coupling to plasma membrane (CAAX) via FKBP |
| 193714 | pB80-hsKIF5B(1-353)GCN4-L-GFP | Expression of dimeric, GFP tagged, human kinesin-1 (KIF5B aa1-353) |
| 193715 | pB80-hsKIF5B(1-353)-GCN4-L-mCherry | Expression of dimeric, mCherry tagged, human kinesin-1 (KIF5B aa1-353) |
| 193716 | pB80-hsKIF5B(1-560)-L-GFP | Expression of GFP tagged human kinesin-1 (KIF5B aa1-560) |
| 193717 | pB80-hsKIF5B(1-560)-Rigor-2xmNeongreen-FRB | Rapalog-inducible coupling to human kinesin-1 (KIF5B aa1-560) via FRB to anchor microtubules |
| 193718 | pB80-hsKIF5B(1-560)Rigor-L-2xHA | Expression of HA tagged human kinesin-1 (KIF5B aa1-560) to anchor microtubules |
| 193719 | pB80-hsKIF5B(1-560)Rigor-L-2xHA-L-FRB | Rapalog-inducible coupling to dimeric human kinesin-1 (KIF5B aa1-560) via FKBP for anterograde transport |
| 193720 | pB80-hsKIF5B(1-560)Rigor-L-mVenus | Expression of mVenus tagged human kinesin-1 (KIF5B aa1-560) to anchor microtubules |
| 193721 | pB80-hsKIF5B(1-560)Rigor-L-mVenus-L-SspB(micro) | Optogenetic coupling to dimeric human kinesin-1 (KIF5B aa1-807) via SSPB(micro) for anterograde transport |