Precise homology-directed installation of large genomic edits in human cells with cleaving and nicking high-specificity Cas9 variants.
Wang Q, Liu J, Janssen JM, Goncalves MAFV
Nucleic Acids Res. 2023 Mar 17:gkad165. doi: 10.1093/nar/gkad165.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 199223 | BB44_pmc.DonorR5.TS | Donor plasmid with CCR5 target sites for ITPN or HMEJ knock-in at the human CCR5 safe harbour locus |
| 199227 | AD59_pEP.DonorCLYBL.TS | Donor plasmid with CLYBL target sites for ITPN or HMEJ knock-in at the human CLYBL safe harbor locus |
| 199228 | AZ64_pE.DonorCLYBL.TS | Donor plasmid with CLYBL target sites for ITPN or HMEJ knock-in at the human CLYBL safe harbor locus |
| 199237 | AM77_pU6.Sa-gRNA.CLYBL | Expression construct encoding a S. aureus guide RNA targeting the human CLYBL safe harbor locus |
| 199238 | AY27_pU6.gRNA.CLYBL | Expression construct encoding a S. pyogenes guide RNA targeting the human CLYBL safe harbor locus |