split-intein Gal4 provides intersectional genetic labeling that is repressible by Gal80.
Ewen-Campen B, Luan H, Xu J, Singh R, Joshi N, Thakkar T, Berger B, White BH, Perrimon N
Proc Natl Acad Sci U S A. 2023 Jun 13;120(24):e2304730120. doi: 10.1073/pnas.2304730120. Epub 2023 Jun 5.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 199195 | pDropIn-T2A-split-intein-Gal4[1-20]-N-int | Insert to create split-intein Gal4[N-int] knock-in vectors via Drop-In cloning, released via BsmBI digest |
| 199196 | pDropIn-T2A-split-intein-Gal4[21-881]-C-int | Insert to create split-intein Gal4[C-int] knock-in vectors via Drop-In cloning, released via BsmBI digest |
| 199197 | pDropIn-T2A-split-intein-Gal4[1-20]-N-int-BlastR | Insert to create split-intein Gal4[N-int] knock-in vectors, with blasticidin resistance, via Drop-In cloning, released via BsmBI digest |
| 199198 | pDropIn-T2A-split-intein-Gal4[21-881]-C-int-G418R | Insert to create split-intein Gal4[21-881]-C-int knock-in vectors, with G418 resistance, via Drop-In cloning, released via BsmBI digest |
| 199199 | pDropIn-T2A-ZpGal4DBD | Insert to create ZpGal4DBD knock-ins, via Drop-In cloning, released via BsmBI digest |
| 199200 | pDropIn-T2A-p65Zp | Insert to create p65Zp knock-ins, via Drop-In cloning, released via BsmBI digest |
| 199201 | pBP-split-intein-Gal4[1-20]-N-int | Gateway destination vector for enhancer-driven split-intein Gal4[1-20]N-int expression |
| 199202 | pBP-split-intein-Gal4[21-881]-C-int | Gateway destination vector for enhancer-driven split-intein Gal4[21-881]C-int expression |
| 199203 | pHD-T2A-split-intein-Gal4[1-20]-N-int | To create "long homology arm" (~1000bp) knock-in donor vectors for split-intein Gal4[1-20]N-int |
| 199204 | pHD-T2A-split-intein-Gal4[21-881]-C-int | To create "long homology arm" (~1000bp) knock-in donor vectors for split-intein Gal4[21-881]C-int |
| 199210 | pTub-split-intein-Gal4[1-20]-N-int | For ubiquitous expression of split-intein Gal4-N-int component under control of the tubulin promoter |
| 199211 | pTub-split-intein-Gal4[21-881]-C-int | For ubiquitous expression of split-intein Gal4-C-int component under control of the tubulin promoter |