A conditional knockout rat resource of mitochondrial protein-coding genes via a DdCBE-induced premature stop codon.
Tan L, Qi X, Kong W, Jin J, Lu D, Zhang X, Wang Y, Wang S, Dong W, Shi X, Chen W, Wang J, Li K, Xie Y, Gao L, Guan F, Gao K, Li C, Wang C, Hu Z, Zhang L, Guo X, Shen B, Ma Y
Sci Adv. 2023 Apr 14;9(15):eadf2695. doi: 10.1126/sciadv.adf2695. Epub 2023 Apr 14.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 198838 | pGL3-MTS-ATP6-W68-L16-G1333C-UGI-Puro | Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ATP6) by swapping Trp codon TGA with stop codon TAA in C6 cells |
| 198839 | pGL3-MTS-ATP6-W68-R17-G1333N-UGI-Puro | Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ATP6) by swapping Trp codon TGA with stop codon TAA in C6 cells |
| 198840 | pGL3-MTS-ATP8-W9-L17-G1333C-UGI-Puro | Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ATP8) by swapping Trp codon TGA with stop codon TAA in C6 cells |
| 198841 | pGL3-MTS-ATP8-W9-R15-G1333N-UGI-Puro | Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ATP8) by swapping Trp codon TGA with stop codon TAA in C6 cells |
| 198842 | pGL3-MTS-COX1-W25-L15-G1333C-UGI-Puro | Employ DdCBE-mediated mtDNA editing to ablate mtProtein(COX1) by swapping Trp codon TGA with stop codon TAA in C6 cells |
| 198843 | pGL3-MTS-COX1-W25-R18-G1333N-UGI-Puro | Employ DdCBE-mediated mtDNA editing to ablate mtProtein(COX1) by swapping Trp codon TGA with stop codon TAA in C6 cells |
| 198844 | pGL3-MTS-COX2-Q6-L18-G1333N-UGI-Puro | Employ DdCBE-mediated mtDNA editing to ablate mtProtein(COX2) by swapping Gln codon CAA with stop codon TAA in C6 cells |
| 198845 | pGL3-MTS-COX2-Q6-R18-G1333C-UGI-Puro | Employ DdCBE-mediated mtDNA editing to ablate mtProtein(COX2) by swapping Gln codon CAA with stop codon TAA in C6 cells |
| 198846 | pGL3-MTS-COX3-W16-L17-G1333C-UGI-Puro | Employ DdCBE-mediated mtDNA editing to ablate mtProtein(COX3) by swapping Trp codon TGA with stop codon TAA in C6 cells |
| 198847 | pGL3-MTS-COX3-W16-R15-G1333N-UGI-Puro | Employ DdCBE-mediated mtDNA editing to ablate mtProtein(COX3) by swapping Trp codon TGA with stop codon TAA in C6 cells |
| 198848 | pGL3-MTS-CYTB-W77-L15-G1333C-UGI-Puro | Employ DdCBE-mediated mtDNA editing to ablate mtProtein(CYTB) by swapping Trp codon TGA with stop codon TAA in C6 cells |
| 198849 | pGL3-MTS-CYTB-W77-R14-G1333N-UGI-Puro | Employ DdCBE-mediated mtDNA editing to ablate mtProtein(CYTB) by swapping Trp codon TGA with stop codon TAA in C6 cells |
| 198850 | pGL3-MTS-ND1-W86-L14-G1333C-UGI-Puro | Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND1) by swapping Trp codon TGA with stop codon TAA in C6 cells |
| 198851 | pGL3-MTS-ND1-W86-R17-G1333N-UGI-Puro | Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND1) by swapping Trp codon TGA with stop codon TAA in C6 cells |
| 198852 | pGL3-MTS-ND2-W30-L16-G1333C-UGI-Puro | Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND2) by swapping Trp codon TGA with stop codon TAA in C6 cells |
| 198853 | pGL3-MTS-ND2-W30-R18-G1333N-UGI-Puro | Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND2) by swapping Trp codon TGA with stop codon TAA in C6 cells |
| 198854 | pGL3-MTS-ND3-Q26-L18-G1397C-UGI-Puro | Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND3) by swapping Gln codon CAA with stop codon TAA in C6 cells |
| 198855 | pGL3-MTS-ND3-Q26-R19-G1397N-UGI-Puro | Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND3) by swapping Gln codon CAA with stop codon TAA in C6 cells |
| 198856 | pGL3-MTS-ND4-W24-L18-G1333C-UGI-Puro | Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND4) by swapping Trp codon TGA with stop codon TAA in C6 cells |
| 198857 | pGL3-MTS-ND4-W24-R17-G1333N-UGI-Puro | Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND4) by swapping Trp codon TGA with stop codon TAA in C6 cells |
| 198858 | pGL3-MTS-ND5-W68-L15-G1333C-UGI-Puro | Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND5) by swapping Trp codon TGA with stop codon TAA in C6 cells |
| 198859 | pGL3-MTS-ND5-W68-R15-G1333N-UGI-Puro | Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND5) by swapping Trp codon TGA with stop codon TAA in C6 cells |
| 198860 | pGL3-MTS-ND6-W82-L15-G1333N-UGI-Puro | Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND6) by swapping Trp codon TGA with stop codon TAA in C6 cells |
| 198861 | pGL3-MTS-ND6-W82-R15-G1333C-UGI-Puro | Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND6) by swapping Trp codon TGA with stop codon TAA in C6 cells |