Genome-wide screen reveals Rab12 GTPase as a critical activator of Parkinson's disease-linked LRRK2 kinase.
Dhekne HS, Tonelli F, Yeshaw WM, Chiang CY, Limouse C, Jaimon E, Purlyte E, Alessi DR, Pfeffer SR
Elife. 2023 Oct 24;12:e87098. doi: 10.7554/eLife.87098.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 198470 | pMCB306-GFP-Rab8A | lentiviral expression of GFP-Rab8A |
| 198471 | pMCB306-GFP-Rab12 | lentiviral expression of GFP-Rab12 |
| 198472 | pMCB306-GFP-Rab29 | lentiviral expression of GFP-Rab29 |
| 198473 | CSII-PPM1H-mApple | Mammalian expression of PPM1H-mApple |
| 198474 | CSII-PPM1H D288A-mApple | Mammalian expression of PPM1H-mApple |
| 198475 | Lenti-guide-puro mRab12-1 | lentiviral stable expression of mRab12 gRNA 1 |
| 198476 | Lenti-guide-puro mRab12-2 | lentiviral stable expression of mRab12 gRNA 2 |
| 198477 | Lenti-guide-puro mAtp6v1a-1 | lentiviral stable expression of mAtp6v1a gRNA 1 |
| 198478 | Lenti-guide-puro mAtp6v1a-2 | lentiviral stable expression of mAtp6v1a gRNA 2 |
| 198479 | Lenti-guide-puro mAtp5c-1 | lentiviral stable expression of mAtp5c gRNA 1 |
| 198480 | Lenti-guide-puro mAtp5c-2 | lentiviral stable expression of mAtp5c gRNA 2 |
| 198481 | Lenti-guide-puro mHgs-1 | lentiviral stable expression of mHgs gRNA 1 |
| 198482 | Lenti-guide-puro mHgs-2 | lentiviral stable expression of mHgs gRNA 2 |
| 198483 | Lenti-guide-puro mPHB2-1 | lentiviral stable expression of mPHB2 gRNA 1 |
| 198484 | Lenti-guide-puro mPHB2-2 | lentiviral stable expression of mPHB2 gRNA 2 |
| 198487 | Lenti-guide-puro mCert1-1 | lentiviral stable expression of mCert1 gRNA 1 |
| 198488 | Lenti-guide-puro mCert1-2 | lentiviral stable expression of mCert1 gRNA 2 |
| 198489 | Lenti-guide-puro mBltp1 (KIAA1109) - 1 | lentiviral stable expression of mBltp1 (KIAA1109) gRNA 1 |
| 198490 | Lenti-guide-puro mBltp1 (KIAA1109) - 2 | lentiviral stable expression of mBltp1 (KIAA1109) gRNA 2 |
| 198491 | Lenti-guide-puro mMyh9 - 1 | lentiviral stable expression of mMyh9 gRNA 1 |
| 198492 | Lenti-guide-puro mMyh9 - 2 | lentiviral stable expression of mMyh9 gRNA 2 |
| 198494 | Lenti-guide-puro mSptlc2 - 2 | lentiviral stable expression of mSptlc2 gRNA 2 |
| 198497 | Lenti-guide-puro mYwhae - 1 | lentiviral stable expression of mYwhae gRNA 1 |
| 198498 | Lenti-guide-puro mYwhae -2 | lentiviral stable expression of mYwhae gRNA 2 |
| 198501 | Lenti-guide-puro mNudcd3 - 1 | lentiviral stable expression of mNudcd3 gRNA 1 |
| 198502 | Lenti-guide-puro mNudcd3 - 2 | lentiviral stable expression of mNudcd3 gRNA 2 |
| 198503 | Lenti-guide-puro mCct8 - 1 | lentiviral stable expression of mCct8 gRNA 1 |
| 198504 | Lenti-guide-puro mCct8 - 2 | lentiviral stable expression of mCct8 gRNA 2 |
| 198505 | Lenti-guide-puro mCsnk2b - 1 | lentiviral stable expression of mCsnk2b gRNA 1 |
| 198506 | Lenti-guide-puro mCsnk2b - 2 | lentiviral stable expression of mCsnk2b gRNA 2 |
| 207333 | pET15b+His SUMO PPM1H | Bacterial expression of His SUMO PPM1H |
| 207334 | pET15b+His SUMO Δ37 PPM1H | Bacterial expression of His SUMO Δ37 PPM1H |
| 207335 | pCMV5D HA-Δ10 PPM1H | Mammalian expression of HA-Δ10 PPM1H |
| 207336 | pCMV5D HA-Δ20 PPM1H | Mammalian expression of HA-Δ20 PPM1H |
| 207337 | pCMV5D HA-Δ34 PPM1H | Mammalian expression of HA-Δ34 PPM1H |
| 207338 | pCMV5D HA-Δ37 PPM1H | Mammalian expression of HA-Δ37 PPM1H |
| 207339 | pCMV5D HA-Δ44 PPM1H | Mammalian expression of HA-Δ44 PPM1H |
| 207340 | pCMV5D HA-Δ71 PPM1H | Mammalian expression of HA-Δ71 PPM1H |
| 207341 | pCMV5D HA-L2D/V9D/I19D PPM1H | Mammalian expression of HA-L2D/V9D/I19D PPM1H |
| 207342 | pET15b+His SUMO Rab10(Q68L) | Bacterial expression of His SUMO Rab10(Q68L) |
| 207343 | pCMV5D HA-Δ115-133, 204-217 PPM1H | Mammalian expression of HA-Δ115-133, 204-217 PPM1H |
| 207344 | pCMV5D HA-Δ37, 115-133, 204-217 PPM1H | Mammalian expression of HA-Δ37, 115-133, 204-217 PPM1H |
| 207345 | pQE-80L 2xHis-LRRK2 Armadillo E240R | Bacterial expression of LRRK2 Armadillo |
| 207346 | pQE-80L 2xHis-LRRK2 Armadillo K439E | Bacterial expression of LRRK2 Armadillo |
| 207347 | pQE-80L 2xHis-Rab12 Q101L | Bacterial expression of Rab12 |
| 207348 | pQE-80L 2xHis-LRRK2 Armadillo F283A | Bacterial expression of LRRK2 Armadillo |
| 207349 | pmcb306-GFP-Rab12-mito | Mammalian expression of Rab12-mito |
| 207350 | CSII-mApple | Mammalian expression of mApple |
| 207351 | CSII-PPM1H-mApple-mito | Mammalian expression of PPm1H-mito |
| 208366 | pcDNA 3.1 myc-Rab10 | Mammalian expression of myc-tagged human Rab10 |
| 208367 | pcDNA 3.1 myc-Rab10-mito | Mammalian expression of myc-tagged human Rab10 with C-terminal mito signal |
| 208368 | pCMV5D HA-PPM1H S124A | Mammalian expression of HA-tagged human PPM1H S124A |
| 208369 | pCMV5D HA-PPM1H S211A | Mammalian expression of HA-tagged human PPM1H S211A |
| 208370 | pCMV5D HA-PPM1H S124A/S211A | Mammalian expression of HA-tagged human PPM1H S124A/S211A |
| 208371 | pET 15b+His SUMO Rab12 (Q101L) | Bacterial expression of His-SUMO-tagged human Rab12 (Q101L) |