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The adaptor protein chaperone AAGAB stabilizes AP-4 complex subunits.

Mattera R, De Pace R, Bonifacino JS
Mol Biol Cell. 2022 Oct 1;33(12):ar109. doi: 10.1091/mbc.E22-05-0177. Epub 2022 Aug 17. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
198327pGADT7-AP-4 β4Expression of GAL4 transcriptional activation domain (AD)-AP-4 β4 fusion protein in yeast (yeast two-hybrid assays)
198328pGAD424-AP-4 σ4Expression of GAL4 transcriptional activation domain (AD)-AP-4 σ4 fusion protein in yeast (yeast two-hybrid assays)
198331pCI-neo-AP-4 (Myc)3-σ4Expresses the triple Myc-tagged mouse AP-4 σ4 subunit in mammalian cells
198332pGBT9-AAGABExpression of GAL4 DNA-binding domain (BD)-AAGAB fusion protein in yeast (yeast two-hybrid assays)
198333pEGFP-N1-AAGABExpresses AAGAB-GFP fusion in mammalian cells
198334pGADT7-AP-1 σ1AExpression of GAL4 transcriptional activation domain (AD)-AP-1 σ1A fusion protein in yeast (yeast two-hybrid assays)
198335pGADT7-AP-1 σ1BExpression of GAL4 transcriptional activation domain (AD)-AP-1 σ1B fusion protein in yeast (yeast two-hybrid assays)
198336pGADT7-AP-1 σ1CExpression of GAL4 transcriptional activation domain (AD)-AP-1 σ1C fusion protein in yeast (yeast two-hybrid assays)
198345pGADT7-AP-2 σ2Expression of GAL4 transcriptional activation domain (AD)-AP-2 σ2 fusion protein in yeast (yeast two-hybrid assays)
198347pACT2-AP-3 σ3AExpression of GAL4 transcriptional activation domain (AD)-AP-3 σ3A fusion protein in yeast (yeast two-hybrid assays)
198348pACT2-AP-3 σ3BExpression of GAL4 transcriptional activation domain (AD)-AP-3 σ3B fusion protein in yeast (yeast two-hybrid assays)

Antibodies from Article