Targeted assembly of ectopic kinetochores to induce chromosome-specific segmental aneuploidies.
Tovini L, Johnson SC, Guscott MA, Andersen AM, Spierings DCJ, Wardenaar R, Foijer F, McClelland SE
EMBO J. 2023 May 15;42(10):e111587. doi: 10.15252/embj.2022111587. Epub 2023 Apr 17.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 198325 | pCENPTΔC-dCas9-3xGFP | Expresses CENPTΔC-dCas9-3xGFP protein in mammalian cells. When coupled with a guide RNA against a high repeat target locus, this can be applied to seed ectopic kinetochores at the target locus |
| 198326 | p-mCherry2-sgC9cen | Expresses a guide RNA against a repetitive locus found in the pericentromere of human chromosome 9, with mCherry2 reporter |
| 198330 | p-mCherry2-sgRNA (empty) | Customisable sgRNA sequence with optimised sgRNA scaffold for expression under U6 promoter, with mCherry2 reporter |
| 198399 | p-mCherry2-sgMUC4 | Expresses a guide RNA against a repetitive locus found in the MUC4 gene of human chromosome 3, with mCherry2 reporter |