An SPNS1-dependent lysosomal lipid transport pathway that enables cell survival under choline limitation.
Scharenberg SG, Dong W, Ghoochani A, Nyame K, Levin-Konigsberg R, Krishnan AR, Rawat ES, Spees K, Bassik MC, Abu-Remaileh M
Sci Adv. 2023 Apr 21;9(16):eadf8966. doi: 10.1126/sciadv.adf8966. Epub 2023 Apr 19.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 198566 | PX458-SPNS1-sg1 | SPNS1-targeting sgRNA in PX458 vector |
| 198567 | PX458-SPNS1-sg2 | SPNS1-targeting sgRNA in PX458 vector |
| 198568 | pMXs-3xFlag-SPNS1 | SPNS1 codon-optimized orf with n-terminal 3xFlag in pMXs-IRES-Blast vector |
| 198569 | pMXs-IRES-blast 3xFlag-SPNS1(E164K) | SPNS1(E164K) codon-optimized orf with n-terminal 3xFlag in pMXs-IRES-Blast vector |
| 198570 | pMXs-IRES-blast 3xFlag-SPNS1(R76A) | SPNS1(R76A) codon-optimized orf with n-terminal 3xFlag in pMXs-IRES-Blast vector |
| 198571 | pMXs-IRES-blast GFP-SPNS1 | SPNS1 codon-optimized orf with n-terminal 3xFlag GFP in pMXs-IRES-Blast vector |
| 198572 | pMXs-IRES-blast GFP-SPNS1(E164K) | SPNS1(E164K) codon-optimized orf with n-terminal 3xFlag GFP in pMXs-IRES-Blast vector |