High-throughput discovery and characterization of viral transcriptional effectors in human cells.

Ludwig CH, Thurm AR, Morgens DW, Yang KJ, Tycko J, Bassik MC, Glaunsinger BA, Bintu L
Cell Syst. 2023 Jun 21;14(6):482-500.e8. doi: 10.1016/j.cels.2023.05.008. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
198054	pCL040 lenti TRE-3xFLAG-LibCloneSite pEF-rTetR(SE-G72P)-VP48-T2A-mCherry-BSD-WPRE	All-in-one lentiviral backbone for doxycycline-inducible expression of a 3xFLAG-tagged protein of interest, which can be inserted with Golden Gate cloning