Direct enzymatic sequencing of 5-methylcytosine at single-base resolution.
Wang T, Fowler JM, Liu L, Loo CE, Luo M, Schutsky EK, Berrios KN, DeNizio JE, Dvorak A, Downey N, Montermoso S, Pingul BY, Nasrallah M, Gosal WS, Wu H, Kohli RM
Nat Chem Biol. 2023 Jun 15. doi: 10.1038/s41589-023-01318-1.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 197985 | pMG81-MBP-t-M.MpeI-N374K-His | Expresses CpG Carboxymethyltransferase (M.MpeI N374K) in bacteria. The gene also encodes an N-terminal (TEV cleavable) MBP tag as well as C-terminal His tag. |