Structures of BIRC6-client complexes provide a mechanism of SMAC-mediated release of caspases.
Hunkeler M, Jin CY, Fischer ES
Science. 2023 Mar 17;379(6637):1105-1111. doi: 10.1126/science.ade5750. Epub 2023 Feb 9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 197967 | pDARMO_CMVT_3xFLAG_BIRC6 | Mammalian cell expression of wt 3xFLAG-BIRC6 |
| 197968 | pDARMO_CMVT_3xFLAG_BIRC6_BIRmut | Mammalian cell expression of 3xFLAG-BIRC6_BIRmut |
| 197969 | pDARMO_CMVT_3xFLAG_BIRC6_delta(Helix) | Mammalian cell expression of 3xFLAG-BIRC6_delta(Helix) |
| 197970 | pDARMO_CMVT_3xFLAG_BIRC6_delta(Helix)_BIRmut | Mammalian cell expression of 3xFLAG-BIRC6_delta(Helix)/BIRmut |
| 197971 | pDARMO_CMVT_N_FLAG_UBA6 | Mammalian cell expression of FLAG-UBA6 |
| 197972 | pDARMO_CMVT_N_Strep_BIRC6 | Mammalian cell expression of wt Strep-BIRC6 |
| 197973 | pDEST_CMV_N_Flag_BIRC6 | Mammalian cell expression of wt FLAG-BIRC6 |
| 197974 | pDONR223_BIRC6 | Entry clone for wt BIRC6 |
| 197975 | pET-20b_HtrA2(delta(133))_S306A_6His | E. coli expression of HtrA2(delta133)-6His |
| 197976 | pNIC-Bio2_SMAC(delta(55))_9His | E. coli expression of SMAC(delta(55))-9His |
| 197977 | pNIC-Bio2_SMAC(delta(55))_Cys_9His | E. coli expression of SMAC(delta(55))_Cys-9His |
| 197978 | pNIC-Bio2_MVPI_SMAC(delta(55))_9His | E. coli expression of MVPI-SMAC(delta(55))_9His |