Optimized incorporation of an unnatural fluorescent amino acid affords measurement of conformational dynamics governing high-fidelity DNA replication.
Dangerfield TL, Johnson KA
J Biol Chem. 2020 Dec 11;295(50):17265-17280. doi: 10.1074/jbc.RA120.015557. Epub 2020 Oct 5.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 197930 | pSUP-(2xCouRS-tRNAopt) | Machinery plasmid expressing constitutive and inducible 7HCou synthetase and cognate amber suppressing tRNA. Incorporates (7-hydroxy-4-coumarin-yl) ethylglycine |