Enhancing stability of recombinant CHO cells by CRISPR/Cas9-mediated site-specific integration into regions with distinct histone modifications.
Hertel O, Neuss A, Busche T, Brandt D, Kalinowski J, Bahnemann J, Noll T
Front Bioeng Biotechnol. 2022 Oct 13;10:1010719. doi: 10.3389/fbioe.2022.1010719. eCollection 2022.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 200009 | pCMV-eGFP-mPlum-HA | Donor vector with double fluorescence system for targeted integration using CRISPR/Cas9. Expresses 5´HA--eGFP&PuroR--3´HA--mPlum. Distinguish targeted from random integration by fluorescence. |