Highly selective transgene expression through the flip-excision switch system by using a unilateral spacer sequence.
Matsushita N, Kato S, Nishizawa K, Sugawara M, Takeuchi K, Miyasaka Y, Mashimo T, Kobayashi K
Cell Rep Methods. 2023 Jan 18;3(2):100393. doi: 10.1016/j.crmeth.2022.100393. eCollection 2023 Feb 27.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 197883 | pAAV-CAGGS-Flex-GFP | Can be used to generate AAV virus that will express GFP in the presence of Cre |
| 197884 | pAAV-CAGGS-Flex/5'USS-GFP | Can be used to generate AAV virus that will express GFP in the presence of Cre and the leakage expression is reduced without Cre |
| 197885 | pAAV-CAGGS-Flex/3'USS-GFP(ATG mut) | Can be used to generate AAV virus that will express GFP in the presence of Cre and the leakage expression is significantly reduced without Cre |
| 197886 | pAAV-CAGGS-Flex-Turbo/GFP | Can be used to generate AAV virus that will express Turbo/GFP in the presence of Cre |
| 197887 | pAAV-CAGGS-Flex/3'USS(Luc)-GFP | Can be used to generate AAV virus that will express GFP in the presence of Cre and the leakage expression is significantly reduced without Cre |
| 197888 | pAAV-CAGGS-Flex/3'USS(553)-GFP | Can be used to generate AAV virus that will express GFP in the presence of Cre including the shortage of a unilateral spacer sequence(USS) |
| 197889 | pAAV-CAGGS-Flex/3'USS(285)-GFP | Can be used to generate AAV virus that will express GFP in the presence of Cre including the shortage of a unilateral spacer sequence(USS) |
| 197890 | pAAV-CAGGS-Flex/3'USS(103)-GFP | Can be used to generate AAV virus that will express GFP in the presence of Cre including the shortage of a unilateral spacer sequence(USS) |
| 197891 | pAAV-EF1α-Flex/3'USS-ChRWR/Venus(ATG mut) | Can be used to generate AAV virus that will express ChRWR/Venus in the presence of Cre and the leakage expression is significantly reduced without Cre |
| 197892 | pAAV-EF1α-Flex/3'USS-hM4D/2A/GFP(ATG mut) | Can be used to generate AAV virus that will express hM4D/2A/GFP in the presence of Cre and the leakage expression is significantly reduced without Cre |
| 197893 | pAAV-EF1α-Flex/3'USS-mCherry(ATG full mut) | Can be used to generate AAV virus that will express mCherry in the presence of Cre and the leakage expression is significantly reduced without Cre |