An expanded CRISPR-Cas9-assisted recombineering toolkit for engineering genetically intractable Pseudomonas aeruginosa isolates.
Pankratz D, Gomez NO, Nielsen A, Mustafayeva A, Gur M, Arce-Rodriguez F, Nikel PI, Haussler S, Arce-Rodriguez A
Nat Protoc. 2023 Oct 5. doi: 10.1038/s41596-023-00882-z.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 197858 | pS148∙CsR | Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria |
| 197859 | pS548∙CsR | Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria |
| 197860 | pS648∙CsR | Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria |
| 198025 | pSH124-ssr | Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E |
| 198026 | pSH424-ssr | Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E |
| 198027 | pSH824-ssr | Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E |
| 199240 | pS848∙CsR | Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria |
| 199241 | pSH624-ssr | Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E |