An extensible vector toolkit and parts library for advanced engineering of plant genomes.
Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R, Gamo ME, Starker CG, Zhang F, Voytas DF
Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
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PubMed
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Article
Some of these materials are included in the Joint Modular Cloning (JMC) Toolkit or the MoClo Plant Parts III: Transformation & Genome Engineering Kit.
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 197688 | pMC-1-EXOB-11 | Phytobrick (MoClo) Level 0 Part |
| 197689 | pMC-1-TBS-11 | Phytobrick (MoClo) Level 0 Part |
| 197690 | pMC-1-Ugi1-11 | Phytobrick (MoClo) Level 0 Part |
| 197691 | pMC-1-Ugi3-11 | Phytobrick (MoClo) Level 0 Part |
| 197692 | pMC-1-Ugi4-11 | Phytobrick (MoClo) Level 0 Part |
| 197693 | pMC-1-Hairpin_Insulator-4 | Phytobrick (MoClo) Level 0 Part |
| 197694 | pMC-1-CmYLCV-4 | Phytobrick (MoClo) Level 0 Part |
| 197695 | pMC-1-DR5v2-4 | Phytobrick (MoClo) Level 0 Part |
| 197696 | pMC-1-OsEf1a-4 | Phytobrick (MoClo) Level 0 Part |
| 197697 | pMC-1-Nos-6 | Phytobrick (MoClo) Level 0 Part |
| 197698 | pMC-1-35S_Long-6 | Phytobrick (MoClo) Level 0 Part |
| 197699 | pMC-1-CmYLCV-6 | Phytobrick (MoClo) Level 0 Part |
| 197700 | pMC-1-AtUbi10-6 | Phytobrick (MoClo) Level 0 Part |
| 197701 | pMC-1-GmHSP17-5E-6 | Phytobrick (MoClo) Level 0 Part |
| 197702 | pMC-1-NtEf1a-6 | Phytobrick (MoClo) Level 0 Part |
| 197703 | pMC-1-OsEf1a-6 | Phytobrick (MoClo) Level 0 Part |
| 197704 | pMC-1-OsUbi-6 | Phytobrick (MoClo) Level 0 Part |
| 197705 | pMC-1-PvUbi1-6 | Phytobrick (MoClo) Level 0 Part |
| 197706 | pMC-1-PvUbi2-6 | Phytobrick (MoClo) Level 0 Part |
| 197707 | pMC-1-SlEf1a-6 | Phytobrick (MoClo) Level 0 Part |
| 197708 | pMC-1-SlRPS5A1-6 | Phytobrick (MoClo) Level 0 Part |
| 197709 | pMC-1-SlUbi-6 | Phytobrick (MoClo) Level 0 Part |
| 197710 | pMC-1-StUbi-6 | Phytobrick (MoClo) Level 0 Part |
| 197711 | pMC-1-ZmAXIG1-6 | Phytobrick (MoClo) Level 0 Part |
| 197712 | pMC-1-ZmEf1a-6 | Phytobrick (MoClo) Level 0 Part |
| 197713 | pMC-1-ZmPLTP-6 | Phytobrick (MoClo) Level 0 Part |
| 197714 | pMC-1-ZmUbi1-6 | Phytobrick (MoClo) Level 0 Part |
| 197715 | pMC-6-BeYDV-Rep-RepA-9 | Phytobrick (MoClo) Level 0 Part |
| 197716 | pMC-6-WDV-Rep-RepA-9 | Phytobrick (MoClo) Level 0 Part |
| 197717 | pMC-1-BeYDV-LS-L-11 | Phytobrick (MoClo) Level 0 Part |
| 197718 | pMC-1-BeYDV-LRS-L-11 | Phytobrick (MoClo) Level 0 Part |
| 197719 | pMC-1-WDV-LS-L-11 | Phytobrick (MoClo) Level 0 Part |
| 197720 | pMC-1-WDV-LRS-L-11 | Phytobrick (MoClo) Level 0 Part |
| 197721 | pMC-6-Cre-9 | Phytobrick (MoClo) Level 0 Part |
| 197722 | pMC-6-Cre-StLS1-9 | Phytobrick (MoClo) Level 0 Part |
| 197723 | pMC-6-Cre-GR-9 | Phytobrick (MoClo) Level 0 Part |
| 197724 | pMC-6-FLPe-9 | Phytobrick (MoClo) Level 0 Part |
| 197725 | pMC-6-PhiC31-9 | Phytobrick (MoClo) Level 0 Part |
| 197726 | pMC-6-R-9 | Phytobrick (MoClo) Level 0 Part |
| 197727 | pMC-6-Integrase4-9 | Phytobrick (MoClo) Level 0 Part |
| 197728 | pMC-6-Integrase13-9 | Phytobrick (MoClo) Level 0 Part |
| 197729 | pMC-6-CinH-9 | Phytobrick (MoClo) Level 0 Part |
| 197730 | pMC-6-Bxb1-9 | Phytobrick (MoClo) Level 0 Part |
| 197731 | pMC-6-AmCyan-9 | Phytobrick (MoClo) Level 0 Part |
| 197732 | pMC-6-Cycle3GFP-9 | Phytobrick (MoClo) Level 0 Part |
| 197733 | pMC-6-EGFP-9 | Phytobrick (MoClo) Level 0 Part |
| 197734 | pMC-6-StayGold-9 | Phytobrick (MoClo) Level 0 Part |
| 197735 | pMC-6-DsRed2-9 | Phytobrick (MoClo) Level 0 Part |
| 197736 | pMC-6-ELuc-9 | Phytobrick (MoClo) Level 0 Part |
| 197737 | pMC-6-FLuc-9 | Phytobrick (MoClo) Level 0 Part |
| 197738 | pMC-6-RedFLuc-9 | Phytobrick (MoClo) Level 0 Part |
| 197739 | pMC-6-RLuc-9 | Phytobrick (MoClo) Level 0 Part |
| 197740 | pMC-6-Ruby-9 | Phytobrick (MoClo) Level 0 Part |
| 197741 | pMC-6-GFP-P2A-NptII-9 | Phytobrick (MoClo) Level 0 Part |
| 197742 | pMC-6-AmCyan-P2A-NptII-9 | Phytobrick (MoClo) Level 0 Part |
| 197743 | pMC-6-HptII-9 | Phytobrick (MoClo) Level 0 Part |
| 197744 | pMC-6-NptII-9 | Phytobrick (MoClo) Level 0 Part |
| 197745 | pMC-6-AtPLT5-9 | Phytobrick (MoClo) Level 0 Part |
| 197746 | pMC-6-IPT-9 | Phytobrick (MoClo) Level 0 Part |
| 197747 | pMC-6-TaGRF-GIF-9 | Phytobrick (MoClo) Level 0 Part |
| 197748 | pMC-6-ZmBbm-9 | Phytobrick (MoClo) Level 0 Part |
| 197749 | pMC-6-ZmWus2-9 | Phytobrick (MoClo) Level 0 Part |
| 197750 | pMC-6-ZmBbm-P2A-ZmWus2-9 | Phytobrick (MoClo) Level 0 Part |
| 197751 | pMC-6-AsNPGA-9 | Phytobrick (MoClo) Level 0 Part |
| 197752 | pMC-6-NnCPH-9 | Phytobrick (MoClo) Level 0 Part |
| 197753 | pMC-6-NnH3H-9 | Phytobrick (MoClo) Level 0 Part |
| 197754 | pMC-6-NnHISPS-9 | Phytobrick (MoClo) Level 0 Part |
| 197755 | pMC-6-NnLuz-9 | Phytobrick (MoClo) Level 0 Part |
| 197756 | pMC-6-HpaB-9 | Phytobrick (MoClo) Level 0 Part |
| 197757 | pMC-6-HpaC-9 | Phytobrick (MoClo) Level 0 Part |
| 197758 | pMC-6-RcTAL-9 | Phytobrick (MoClo) Level 0 Part |
| 197759 | pMC-9-35S-11 | Phytobrick (MoClo) Level 0 Part |
| 197760 | pMC-9-AtHSP-11 | Phytobrick (MoClo) Level 0 Part |
| 197761 | pMC-9-NbAct3-11 | Phytobrick (MoClo) Level 0 Part |
| 197762 | pMC-9-NbHSP-11 | Phytobrick (MoClo) Level 0 Part |
| 197763 | pMC-9-NtEU-11 | Phytobrick (MoClo) Level 0 Part |
| 197764 | pMC-9-NtEf1a-11 | Phytobrick (MoClo) Level 0 Part |
| 197765 | pMC-9-OsAct2-11 | Phytobrick (MoClo) Level 0 Part |
| 197766 | pMC-9-OsEf1a-11 | Phytobrick (MoClo) Level 0 Part |
| 197767 | pMC-9-OsT28-11 | Phytobrick (MoClo) Level 0 Part |
| 197768 | pMC-9-OsUbi-11 | Phytobrick (MoClo) Level 0 Part |
| 197769 | pMC-9-PinII-11 | Phytobrick (MoClo) Level 0 Part |
| 197770 | pMC-9-RBCS-11 | Phytobrick (MoClo) Level 0 Part |
| 197771 | pMC-9-SlEf1a-11 | Phytobrick (MoClo) Level 0 Part |
| 197772 | pMC-9-SlRPS5A1-11 | Phytobrick (MoClo) Level 0 Part |
| 197773 | pMC-9-SlUbi-11 | Phytobrick (MoClo) Level 0 Part |
| 197774 | pMC-9-StUbi-11 | Phytobrick (MoClo) Level 0 Part |
| 197775 | pMC-9-ZmEf1a-11 | Phytobrick (MoClo) Level 0 Part |
| 197776 | pMC-9-ZmIn2-11 | Phytobrick (MoClo) Level 0 Part |
| 197777 | pMC-9-ZmUbi-11 | Phytobrick (MoClo) Level 0 Part |
| 197778 | pMC-6-AtSpCas9-9 | Phytobrick (MoClo) Level 0 Part |
| 197779 | pMC-1-AtU6-Y | Phytobrick (MoClo) Level 0 Part |
| 197780 | pMC-1-OsU6-X | Phytobrick (MoClo) Level 0 Part |
| 197781 | pMC-Z-SpCas9-gRNA-repeat-polyT-11 | Phytobrick (MoClo) Level 0 Part |
| 197782 | pMIN-0 | Level 2 destination vector: minimal (non-T-DNA) backbone |
| 197783 | pMIN-VS1 | Level 2 destination vector: pMIN-VS1 backbone |
| 197784 | pMIN-Ri | Level 2 destination vector: pMIN-Ri backbone |
| 197785 | pJMC-1T | Level 1 Module 1T destination vector |
| 197786 | pJMC-1 | Level 1 Module 1 destination vector |
| 197787 | pJMC-2T | Level 1 Module 2T destination vector |
| 197788 | pJMC-2 | Level 1 Module 2 destination vector |
| 197789 | pJMC-3T | Level 1 Module 3T destination vector |
| 197790 | pJMC-3 | Level 1 Module 3 destination vector |
| 197791 | pJMC-4T | Level 1 Module 4T destination vector |
| 197792 | pJMC-4 | Level 1 Module 4 destination vector |
| 197793 | pJMC-5T | Level 1 Module 5T destination vector |
| 197794 | pJMC-5 | Level 1 Module 5 destination vector |
| 197795 | pJMC-6T | Level 1 Module 6T destination vector |
| 197796 | pJMC-6 | Level 1 Module 6 destination vector |
| 197797 | pJMC-7T | Level 1 Module 7T destination vector |
| 197798 | pJMC-1T-Reverse | Level 1 Module 1T destination vector (reverse orientation MoClo insert) |
| 197799 | pJMC-1-Reverse | Level 1 Module 1 destination vector (reverse orientation MoClo insert) |
| 197800 | pJMC-2T-Reverse | Level 1 Module 2T destination vector (reverse orientation MoClo insert) |
| 197801 | pJMC-2-Reverse | Level 1 Module 2 destination vector (reverse orientation MoClo insert) |
| 197802 | pJMC-3T-Reverse | Level 1 Module 3T destination vector (reverse orientation MoClo insert) |
| 197803 | pJMC-3-Reverse | Level 1 Module 3 destination vector (reverse orientation MoClo insert) |
| 197804 | pJMC-4T-Reverse | Level 1 Module 4T destination vector (reverse orientation MoClo insert) |
| 197805 | pJMC-4-Reverse | Level 1 Module 4 destination vector (reverse orientation MoClo insert) |
| 197806 | pJMC-5T-Reverse | Level 1 Module 5T destination vector (reverse orientation MoClo insert) |
| 197807 | pJMC-5-Reverse | Level 1 Module 5 destination vector (reverse orientation MoClo insert) |
| 197808 | pJMC-6T-Reverse | Level 1 Module 6T destination vector (reverse orientation MoClo insert) |
| 197809 | pJMC-6-Reverse | Level 1 Module 6 destination vector (reverse orientation MoClo insert) |
| 197810 | pJMC-7T-Reverse | Level 1 Module 7T destination vector (reverse orientation MoClo insert) |
| 197811 | pMC-1-ccdB-6 | Level 0 J1-J6 destination vector, BbsI entry & CcdB selection |
| 197812 | pMC-6-ccdB-9 | Level 0 J6-J9 destination vector, BbsI entry & CcdB selection |
| 197813 | pMC-9-ccdB-11 | Level 0 J9-J11 destination vector, BbsI entry & CcdB selection |
| 197814 | pMC-1-ccdB-4 | Level 0 J1-J4 destination vector, BbsI entry & CcdB selection |
| 197815 | pMC-1-ccdB-11 | Level 0 J1-J11 destination vector, BbsI entry & CcdB selection |
| 197816 | pJMC-1-BsaI | Level 1 module 1 with BsaI expansion point |
| 197817 | pJMC-2T-BsaI | Level 1 module 2T with BsaI expansion point |
| 197818 | pJMC-2-BsaI | Level 1 module 2 with BsaI expansion point |
| 197819 | pJMC-3T-BsaI | Level 1 module 3T with BsaI expansion point |
| 197820 | pJMC-3-BsaI | Level 1 module 3 with BsaI expansion point |
| 197821 | pJMC-4T-BsaI | Level 1 module 4T with BsaI expansion point |
| 197822 | pJMC-4-BsaI | Level 1 module 4 with BsaI expansion point |
| 197823 | pJMC-5T-BsaI | Level 1 module 5T with BsaI expansion point |
| 197824 | pJMC-5-BsaI | Level 1 module 5 with BsaI expansion point |
| 197825 | pJMC-6T-BsaI | Level 1 module 6T with BsaI expansion point |
| 197826 | pJMC-6-BsaI | Level 1 module 6 with BsaI expansion point |
| 197827 | pJMC-7T-BsaI | Level 1 module 7T with BsaI expansion point |
| 197828 | pJMC-1-Esp3I | Level 1 module 1 with Esp3I expansion point |
| 197829 | pJMC-2T-Esp3I | Level 1 module 2T with Esp3I expansion point |
| 197830 | pJMC-2-Esp3I | Level 1 module 2 with Esp3I expansion point |
| 197831 | pJMC-3T-Esp3I | Level 1 module 3T with Esp3I expansion point |
| 197832 | pJMC-3-Esp3I | Level 1 module 3 with Esp3I expansion point |
| 197833 | pJMC-4T-Esp3I | Level 1 module 4T with Esp3I expansion point |
| 197834 | pJMC-4-Esp3I | Level 1 module 4 with Esp3I expansion point |
| 197835 | pJMC-5T-Esp3I | Level 1 module 5T with Esp3I expansion point |
| 197836 | pJMC-5-Esp3I | Level 1 module 5 with Esp3I expansion point |
| 197837 | pJMC-6T-Esp3I | Level 1 module 6T with Esp3I expansion point |
| 197838 | pJMC-6-Esp3I | Level 1 module 6 with Esp3I expansion point |
| 197839 | pJMC-7T-Esp3I | Level 1 module 7T with Esp3I expansion point |
| 197840 | pJMC-L2-Donor-BsaI | Donor vector to restore L2 destination via BsaI |
| 197841 | pJMC-L2-Donor-Esp3I | Donor vector to restore L2 destination via Esp3I |
| 197842 | pJMC-L1-Donor-Esp3I | Donor vector to restore L1 destination via Esp3I |
| 197843 | pJMC-L2-Reverse-Donor-BsaI | Donor vector to restore L2 destination via BsaI (reverse orientation) |
| 197844 | pJMC-L2-Reverse-Donor-Esp3I | Donor vector to restore L2 destination via Esp3I (reverse orientation) |
| 197845 | pJMC-L1-Reverse-Donor-Esp3I | Donor vector to restore L1 destination via Esp3I (reverse orientation) |
| 197846 | pJMC-1-Empty | Level 1 module 1 empty |
| 197847 | pJMC-2T-Empty | Level 1 module 2T empty |
| 197848 | pJMC-2-Empty | Level 1 module 2 empty |
| 197849 | pJMC-3T-Empty | Level 1 module 3T empty |
| 197850 | pJMC-3-Empty | Level 1 module 3 empty |
| 197851 | pJMC-4T-Empty | Level 1 module 4T empty |
| 197852 | pJMC-4-Empty | Level 1 module 4 empty |
| 197853 | pJMC-5T-Empty | Level 1 module 5T empty |
| 197854 | pJMC-5-Empty | Level 1 module 5 empty |
| 197855 | pJMC-6T-Empty | Level 1 module 6T empty |
| 197856 | pJMC-6-Empty | Level 1 module 6 empty |
| 197857 | pJMC-7T-Empty | Level 1 module 7T empty |