P1 Bacteriophage-Enabled Delivery of CRISPR-Cas9 Antimicrobial Activity Against Shigella flexneri.
Huan YW, Torraca V, Brown R, Fa-Arun J, Miles SL, Oyarzun DA, Mostowy S, Wang B
ACS Synth Biol. 2023 Mar 17;12(3):709-721. doi: 10.1021/acssynbio.2c00465. Epub 2023 Feb 20.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 197871 | pBBR1.cas9-NT | Constitutive expression of Cas9 endonuclease, trancRNA and crRNA with a randomly generated spacer sequence (non-targeting) |