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Improving target amino acid selectivity in a permissive aminoacyl tRNA synthetase through counter-selection.

Sungwienwong I, Hostetler ZM, Blizzard RJ, Porter JJ, Driggers CM, Mbengi LZ, Villegas JA, Speight LC, Saven JG, Perona JJ, Kohli RM, Mehl RA, Petersson EJ
Org Biomol Chem. 2017 May 3;15(17):3603-3610. doi: 10.1039/c7ob00582b. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
197651pDule- Mj Acd A9E. coli machinery vector for the genetic encoding of acridone at TAG codons using the M. jannaschii Acd-A9 synthetase/tRNA pair
197652pDule2 - Mj Acd A9E. coli machinery vector for the genetic encoding of acridone at TAG codons using the M. jannaschii Acd-A9 synthetase/tRNA pair

Antibodies from Article