Rapid and reversible optogenetic silencing of synaptic transmission by clustering of synaptic vesicles.
Vettkotter D, Schneider M, Goulden BD, Dill H, Liewald J, Zeiler S, Guldan J, Ates YA, Watanabe S, Gottschalk A
Nat Commun. 2022 Dec 19;13(1):7827. doi: 10.1038/s41467-022-35324-z.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 197596 | pDV12 [psng-1::SNG-1::CRY2olig(535)] | Pan-neuronal expression of SNG-1::CRY2olig(535) in neurons of C. elegans |
| 197597 | pDV06 [punc-17::SNG-1::CRY2(535)] | Expression of SNG-1::CRY2olig(535) in cholinergic motor neurons of C. elegans |
| 197598 | pDV18 [punc-47::SNG-1::CRY2olig(535)] | Expression of SNG-1::CRY2olig(535) in GABAergic motor neurons of C. elegans |
| 197599 | pDV21 [pSNG-1::SNG-1::CRY2(D387A)olig(535)::SL2::mCherry] | Pan-neuronal expression of SNG-1::CRY2(D387A)olig(535) in neurons of C. elegans. CRY2(D387A) is photoinactive |
| 198381 | pHD57 [Tol2-UAS:sypb-egfp-cry2-polyA] | Expression of SYPB::CRY2olig(535) in neurons of Danio rerio |