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An engineered bacterial symbiont allows noninvasive biosensing of the honey bee gut environment.

Chhun A, Moriano-Gutierrez S, Zoppi F, Cabirol A, Engel P, Schaerli Y
PLoS Biol. 2024 Mar 5;22(3):e3002523. doi: 10.1371/journal.pbio.3002523. eCollection 2024 Mar. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
197400pAC04Honey bee gut symbiont broad-host range vector with constitutive E2-crimson, specR and pBBR1 replicon
197401pAC06Honey bee gut symbiont broad-host range vector with constitutive E2-crimson, specR and RK2 replicon
197402pAC08Honey bee gut symbiont broad-host range vector with constitutive GFP, ampR and RSF1010 replicon
197403pAC09Honey bee gut symbiont broad-host range vector with constitutive E2-crimson, ampR and RSF1010 replicon
197404pAC10Honey bee gut symbiont broad-host range vector with constitutive E2-crimson, specR and pVS1 replicon
197405pAC11Honey bee gut symbiont broad-host range vector with constitutive E2-crimson, specR and pTFC-FC2 replicon
197406pAC12Honey bee gut symbiont broad-host range vector with constitutive GFP, ampR and pBBR1 replicon
197407pAC13Honey bee gut symbiont broad-host range vector with constitutive GFP, ampR and pVS1 replicon
197408pAC14Honey bee gut symbiont broad-host range vector with constitutive GFP, ampR and pTFC-FC2 replicon
197409pAC23Honey bee gut symbiont broad-host range vector with constitutive E2-crimson, ampR and pBBR1 replicon
197410pAC24Honey bee gut symbiont broad-host range vector with constitutive E2-crimson, ampR and pTFC-FC2 replicon
197411pAC25Honey bee gut symbiont broad-host range vector with constitutive E2-crimson, ampR and RK2 replicon
197412pAC26Honey bee gut symbiont broad-host range vector with constitutive GFP, ampR and RK2 replicon
197413pAC17V5aIPTG-inducible two-plasmid system bearing CP25 > lacO-GFP, specR, RSF1010 replicon (1/2)
197414pAC17V5bIPTG-inducible two-plasmid system bearing CP25 > lacI, ampR, pTF-FC2 replicon (2/2)

Antibodies from Article