Stomach-derived human insulin-secreting organoids restore glucose homeostasis.
Huang X, Gu W, Zhang J, Lan Y, Colarusso JL, Li S, Pertl C, Lu J, Kim H, Zhu J, Breault DT, Sevigny J, Zhou Q
Nat Cell Biol. 2023 Apr 27. doi: 10.1038/s41556-023-01130-y.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 197385 | pLenti-EF1a-Ngn3ER-PuroR-mCherry | To establish Ngn3ER cell line for endocrine differentiation (4-hydroxytamoxifen activation, Puromycin resistant) |
| 197386 | pLenti-CMV-PM | To constitutively express Pdx1-Mafa and in cells (Blasticidin resistant). |
| 197387 | pLenti-TetO-PM | To establish all-in-one TetO-inducible cell line for Pdx1-Mafa expression (Doxycycline induced; rtTA included; Blasticidin resistant) |