Synaptic vesicle proteins are selectively delivered to axons in mammalian neurons.

Watson ET, Pauers MM, Seibert MJ, Vevea JD, Chapman ER
Elife. 2023 Feb 2;12:e82568. doi: 10.7554/eLife.82568. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
197275	pFsynW SYT1 reporter	Encodes for viral expression of wild type Synaptotagmin 1 (SYT1) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).
197276	pFsynW SYB2 reporter	Encodes for viral expression of wild type Synaptobrevin 2 (SYB2) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).
197277	pFsynW KDEL Hook	Encodes ER-targeted streptavidin-KDEL hook (RUSH system).
197278	pFsynW SYT1-PGM reporter	Encodes for viral expression of Synaptotagmin 1 (SYT1) for use in the RUSH system; visualized with HaloTag. Reporter only (no hook). SYT1 is mutated to disrupt palmitoylation and glycosylation.
197279	pFsynW SYT1deltaC2AB reporter	Encodes for viral expression of Synaptotagmin 1 (SYT1) (1 - 140 a.a.) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).
197280	pEF HaloTag-SYT1	Encodes full-length wild type synaptotagmin 1 (SYT1) with an N-terminal HaloTag
197281	pEF SYT1-HaloTag	Encodes full-length wild type synaptotagmin 1 (SYT1) with a C-terminal HaloTag
197282	pEF HaloTag-SYB2	Encodes full-length Synaptobrevin 2 (SYB2) with mutations Q76V and F77W to confer resistance to tetanus cleavage.
197283	pFsynW SYP-GFP	Encodes for viral expression of full-length wild type synaptophysin (SYP). Visualized by a C-terminal GFP.
197284	pFsynW TeTx-LC	Encodes for viral expression of the light chain of tetanus toxin.