A multi-kingdom genetic barcoding system for precise target clone isolation
Ishiguro S, Ishida K, Sakata RC, Mori H, Takana M, King S, Bashth O, Ichiraku M, Masuyama N, Takimoto R, Kijima Y, Adel A, Toyoshima H, Seki M, Oh JH, Archambault AS, Nishida K, Kondo A, Kuhara S, Aburatani H, Klein Geltink RI, Takashima Y, Shakiba N, Yachie N
bioRxiv 2023.01.18.524633
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 197133 | pLV-CS-060 | CRISPRa reporter cloning backbone and MCP-p65-HSF1-2A-Puro expression |
| 197134 | pLV-CS-117 | CloneSelect C-to-T reporter encoding three target barcodes (3-input OR gate circuit) |
| 197135 | pLV-CS-119 | CloneSelect A-to-G reporter encoding three target barcodes (3-input AND gate circuit) |
| 197136 | pLV-CS-146 | CloneSelect Indel reporter encoding BC-del1 barcode |
| 197137 | pLV-CS-193 | CloneSelect C-to-T mCherry (M1V/M9A) reporter encoding BC-C1 barcode |
| 197138 | pLV-CS-196 | CloneSelect C-to-T mCherry (M9A) ATG control reporter encoding BC-C1 barcode |
| 197139 | pSI-218 | All-in-vector of SpCas9 gRNA cloning sites (BsmBI) and CMVp-Target-AID expression unit |
| 197140 | pRS191 | scCloneSelect C-to-T reporter encoding BC-C1 barcode (EF1a promoter version) |
| 197141 | pRS140 | scCloneSelect C-to-T reporter encoding BC-C1 barcode (CMV promoter version) |
| 197142 | pRS138 | scCloneSelect C-to-T DNA barcode cloning backbone (CMV promoter version) |
| 197143 | pRS193 | scCloneSelect C-to-T DNA barcode cloning backbone (EF1a promoter version) |
| 197144 | pNM1325 | CAGp-Target-AID-2A-BlastR for PiggyBac genomic integration |