Decoding CAR T cell phenotype using combinatorial signaling motif libraries and machine learning.
Daniels KG, Wang S, Simic MS, Bhargava HK, Capponi S, Tonai Y, Yu W, Bianco S, Lim WA
Science. 2022 Dec 16;378(6625):1194-1200. doi: 10.1126/science.abq0225. Epub 2022 Dec 8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 197081 | pMAT_M1_YLVV | This plasmid contains the coding sequence for signaling peptide M1, which contains the YLVV signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP |
| 197082 | pMAT_M2_YVPM | This plasmid contains the coding sequence for signaling peptide M2, which contains the YVPM signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP |
| 197083 | pMAT_M3_YENL | This plasmid contains the coding sequence for signaling peptide M3, which contains the YENL signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP |
| 197084 | pMAT_M4_YVTM_YQNQ | This plasmid contains the coding sequence for signaling peptide M4, which contains the YVTM and YQNQ signaling motifs. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP |
| 197085 | pMAT_M5_YESP | This plasmid contains the coding sequence for signaling peptide M5, which contains the YESP signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP |
| 197086 | pMAT_M6_ITYAAV | This plasmid contains the coding sequence for signaling peptide M6, which contains the ITYAAV signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP |
| 197087 | pMAT_M7_PVQP | This plasmid contains the coding sequence for signaling peptide M7, which contains the PVQP signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP |
| 197088 | pMAT_M8_RSTKP | This plasmid contains the coding sequence for signaling peptide M8, which contains the RSTKP signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP |
| 197091 | pMAT_M9_PVQE | This plasmid contains the coding sequence for signaling peptide M9, which contains the PVQE signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP |
| 197093 | pMAT_M10_PQQAT | This plasmid contains the coding sequence for signaling peptide M10, which contains the PQQAT signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP |
| 197094 | pMAT_M11_PQEINF | This plasmid contains the coding sequence for signaling peptide M11, which contains the PQEINF signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP |
| 197095 | pMAT_M12_RPTAVEG | This plasmid contains the coding sequence for signaling peptide M12, which contains the RPTAVEG signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP |
| 197096 | pMAT_M13_6xSAG | This plasmid contains the coding sequence for the control peptide M13, which contains a linker with six repeats of serine-alanine-glycine. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_C |
| 197097 | pMAT_41BBICD | This plasmid contains the coding sequence for the intracellular domain of 4-1BB. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP |
| 197098 | pMAT_CD28ICD | This plasmid contains the coding sequence for the intracellular domain of CD28. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP |