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An improved imaging system that corrects MS2-induced RNA destabilization.

Li W, Maekiniemi A, Sato H, Osman C, Singer RH
Nat Methods. 2022 Dec;19(12):1558-1562. doi: 10.1038/s41592-022-01658-1. Epub 2022 Nov 10. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
197047pWL078 MCP-2xGFPpCYC-NLS-MCP-2xGFP on pRS305
197052yWL268 MCP-GFPpUBC-NLS-HA-2xMCP-2xGFP
197053yWL229 MCP-GFP-eRF3pUBC-NLS-HA-2xMCP-1xGFP-eRF3
197054yWL234 MCP-GFP-PABPC1*pUBC-NLS-HA-2xMCP-1xGFP-PABPC1*

Antibodies from Article