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Monitoring the 5-methoxycarbonylmethyl-2-thiouridine (mcm5s2U) modification in eukaryotic tRNAs via the gamma-toxin endonuclease.

Lentini JM, Ramos J, Fu D
RNA. 2018 May;24(5):749-758. doi: 10.1261/rna.065581.118. Epub 2018 Feb 13. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
197000pET28-His10Smt3-gamma-toxinBacterial expression plasmid for gamma toxin tRNA endonuclease

Antibodies from Article