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Heritable transgene-free genome editing in plants by grafting of wild-type shoots to transgenic donor rootstocks.

Yang L, Machin F, Wang S, Saplaoura E, Kragler F
Nat Biotechnol. 2023 Jan 2. doi: 10.1038/s41587-022-01585-8. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
196977Cas9 TLS1Expresses Cas9 fused with TLS1 mobility signal that confers graft mobility. Induced by estradiol.
196978Cas9 TLS2Expresses Cas9 fused with TLS2 mobility signal that confers graft mobility. Induced by estradiol.
196980gRNA NIA TLS1Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Each gRNA is fused to TLS1 mobility signal that confers graft mobility.
196981gRNA NIA TLS2Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Each gRNA is fused to TLS2 mobility signal that confers graft mobility.
196983Fragment1 with TLS2Gateway-compatible gRNA backbone with TLS2 mobility signal. Template for the addition of target sequences to produce 2x graft-mobile gRNA-TLS2.
198727Cas9 NullExpresses Cas9 without a mobility signal and the mRNA is not graft mobile. Induced by estradiol.
198728gRNA NIA NullExpresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Neither gRNA is fused to a mobility signal and are therefore non-mobile.
198729Fragment 1 NullGateway-compatible gRNA backbone . Template for the addition of target sequences to produce 2x gRNA.

Antibodies from Article