Genetically encoded multimeric tags for subcellular protein localization in cryo-EM.
Fung HKH, Hayashi Y, Salo VT, Babenko A, Zagoriy I, Brunner A, Ellenberg J, Muller CW, Cuylen-Haering S, Mahamid J
Nat Methods. 2023 Dec;20(12):1900-1908. doi: 10.1038/s41592-023-02053-0. Epub 2023 Nov 6.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 197056 | pcDNA3.1_GEM2-Halo-FRB | Expression of Synechococcus elongatus encapsulin Halo-FRB-fusion in mammalian cells |
| 197057 | pcDNA3.1_GEM4-Halo-FRB | Expression of Paraburkholderia xenovorans encapsulin Halo-FRB-fusion in mammalian cells |
| 197058 | pcDNA3.1_GEM7-Halo-FRB | Expression of Rhodospirillum rubrum encapsulin Halo-FRB-fusion in mammalian cells |
| 197059 | pcDNA3.1_GEM22-Halo-FRB | Expression of Mycobacterium hassiacum encapsulin Halo-FRB-fusion in mammalian cells |
| 197060 | pcDNA3.1_FRB-GEM23-Halo | Expression of nanocage I3-01 Halo-FRB-fusion in mammalian cells |
| 197061 | pAAVS1_TREtight-GEM2-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1 | Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM2-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4) |
| 197062 | pAAVS1_TREtight-GEM4-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1 | Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM4-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4) |
| 197063 | pAAVS1_TREtight-GEM7-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1 | Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM7-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4) |
| 197064 | pAAVS1_TREtight-GEM22-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1 | Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM22-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4) |
| 197065 | pAAVS1_TREtight-FRB-GEM23-Halo-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1 | Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of FRB-GEM23-Halo and Adaptor Protein (FKBP-SNAP-vhhGFP4) |
| 197066 | pAAVS1_EF1a-GEM2-Halo-FKBP-IRES-Blast | Donor plasmid for human AAVS1 locus knock-in, constitutive expression of GEM2-Halo-FKBP |
| 197067 | pAAVS1_EF1a-MitoTrap-mCherry-IRES-Puro | Donor plasmid for human AAVS1 locus knock-in, constitutive expression of MitoTrap-mCherry |