Non-canonical beta-adrenergic activation of ERK at endosomes.

Kwon Y, Mehta S, Clark M, Walters G, Zhong Y, Lee HN, Sunahara RK, Zhang J
Nature. 2022 Nov;611(7934):173-179. doi: 10.1038/s41586-022-05343-3. Epub 2022 Oct 26. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
205519	pcDNA3-nuc-Nb80	Nuclear-targeted Nb80 nanobody that binds and stabilizes β2AR active conformation, blocking Gαs coupling.
205518	pcDNA3-endo-Nb80	Endosome-targeted Nb80 nanobody that binds and stabilizes β2AR active conformation, blocking Gαs coupling.
205517	pcDNA3-nuc-GsCT	Nuclear-targeted C-terminal fragment of human Gαs protein (Gαs inhibitory peptide).
205516	pcDNA3-endo-GsCT	Endosome-targeted C-terminal fragment of human Gαs protein (Gαs inhibitory peptide).
205515	pcDNA3-nuc-Nb37	Nuclear-targeted Nb37 nanobody that binds and stabilizes the active form of Gαs G-protein subunit.
205514	pcDNA3-endo-Nb37	Endosome-targeted Nb37 nanobody that binds and stabilizes the active form of Gαs G-protein subunit.
205513	pcDNA3-endo-EKAR4	Genetically encoded FRET-based biosensor for monitoring ERK kinase activity. Targeted to endosomes.