Non-canonical beta-adrenergic activation of ERK at endosomes.
Kwon Y, Mehta S, Clark M, Walters G, Zhong Y, Lee HN, Sunahara RK, Zhang J
Nature. 2022 Nov;611(7934):173-179. doi: 10.1038/s41586-022-05343-3. Epub 2022 Oct 26.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 205513 | pcDNA3-endo-EKAR4 | Genetically encoded FRET-based biosensor for monitoring ERK kinase activity. Targeted to endosomes. |
| 205514 | pcDNA3-endo-Nb37 | Endosome-targeted Nb37 nanobody that binds and stabilizes the active form of Gαs G-protein subunit. |
| 205515 | pcDNA3-nuc-Nb37 | Nuclear-targeted Nb37 nanobody that binds and stabilizes the active form of Gαs G-protein subunit. |
| 205516 | pcDNA3-endo-GsCT | Endosome-targeted C-terminal fragment of human Gαs protein (Gαs inhibitory peptide). |
| 205517 | pcDNA3-nuc-GsCT | Nuclear-targeted C-terminal fragment of human Gαs protein (Gαs inhibitory peptide). |
| 205518 | pcDNA3-endo-Nb80 | Endosome-targeted Nb80 nanobody that binds and stabilizes β2AR active conformation, blocking Gαs coupling. |
| 205519 | pcDNA3-nuc-Nb80 | Nuclear-targeted Nb80 nanobody that binds and stabilizes β2AR active conformation, blocking Gαs coupling. |