Simultaneous multifunctional transcriptome engineering by CRISPR RNA scaffold.
Liu Z, Jillette N, Robson P, Cheng AW
Nucleic Acids Res. 2023 Aug 11;51(14):e77. doi: 10.1093/nar/gkad547.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 196827 | pzk228-pmax-SgrAI[3xFLAG]-AscI-dPspCas13b(AAAA) | Express dpdpCas13b with AAAA mutation |
| 196828 | pzk408-pmax-3xflag-dpspCas13b(AAAA)-NES | Express dpdpCas13b with AAAA mutation |
| 196829 | pmax-3xFLAG_3xNLS_dPspCas13b(AAAA)_2xNLS-Clover | Express dpdpCas13b with AAAA mutation and Clover |
| 196830 | pmax-hcoRBFOX1N-PUFc-RBFOX1C | Express hcoRBFOX1N-PUFc-RBFOX1C(190-397) |
| 196831 | pzk384-pmax-PUFc-FseI-RESCUE-SalI-NES | Express PUFc-RESCUE-S |
| 196837 | pmax-PUFc_NES-ADARdd(E488Q) | Express PUFc_NES-ADARdd(E488Q) |
| 196838 | pmax-hcoRBFOX1N-PCP-RBFOX1C | Express hcoRBFOX1N-PCP-RBFOX1C(190-397) |
| 196839 | pmax-hcoRBFOX1N-MCP-RBFOX1C | Express hcoRBFOX1N-MCP-RBFOX1C(190-397) |
| 196840 | pmax-MCP_NES-ADARdd(E488Q) | Express MCP_NES-ADARdd(E488Q) |
| 196841 | pzk488-pmax-NES-PUFc-FseI[ADAR2-DD-splitN] | Express NES-PUFc-FseI[ADAR2-DD-splitN] |
| 196842 | pzk491-pmax-NES-PUFc-FseI[ADAR2-DD-splitC] | Express NES-PUFc-FseI[ADAR2-DD-splitC] |
| 196843 | pzk490-pmax-NES-dpspCas13b(AAAA)-FseI[ADAR2-DD-splitC] | Express NES-dpspCas13b(AAAA)-FseI[ADAR2-DD-splitC] |
| 196844 | gzk328-dpspCas13b_gRNA[ccdbCam]-10xPBSc_Loop | backbone for gRNA cloning of dpspCas13b tagged by 10xPBSc_Loop |
| 196845 | gzk326-dpspCas13b_gRNA[ccdbCam]-5xPBSc_Loop | backbone for gRNA cloning of dpspCas13b tagged by 5xPBSc_Loop |
| 196846 | pCR8-PspCas13b_gRNA[ccdbCam]-2xPP7 | backbone for gRNA cloning of dpspCas13b tagged by 2xPP7 |
| 196847 | pCR8-PspCas13b_gRNA[ccdbCam]_1xMS2b | backbone for gRNA cloning of dpspCas13b tagged by 1xMS2 |