Bernhard Schmierer Lab: Schmierer unpublished plasmids
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 196711 | lenti-dCas9-VP64-BFP | CRISPR-activation. dCas9-VP64 with TagBFP for sorting |
| 196712 | lenti-dCas9-ZIM3-KRAB-BFP | CRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3 |
| 196714 | lenti.Cas9.BFP.Blast | WT SpCas9 with BSD-TagBFP for selection and sorting |
| 196715 | lenti.Cas9.BFP.sgRNA.parental | Sortable WT-SpCas9 expression and guide cassette (1-vector system). |
| 196716 | lenti-UCOE-dCas9-BFP-Zim3-KRAB | CRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3 |
| 196717 | lenti-UCOE-dCas9-BFP-VP64 | CRISPR-activation. dCas9-VP64 with TagBFP for sorting. |
| 196718 | CROPSeq-BFP | CROP-Seq library plasmid with Chen tracrRNA (PMID: 24360272) and TagBFP |
| 196719 | CROPSeq-PP7-p65-HSF | CROP-Seq library plasmid for CRISPR-A with SAM system (p65-HSF1 activator recruited to PP7 binding loops in the tracrRNA of the guide). |
| 196720 | pCW-Tet-Cas9-BFP-PGK-Blast | Tet-inducible expression of Cas9-T2A-TagBFP |
| 196721 | lenti-Cas9-Exo-Blast-BFP | Sortable and selectable SpCas9 fused to exodeoxyribonuclease I (sbcB) from E. coli. For the creation of longer deletions. |
| 196722 | pCW-Tet-Cas9-miRFP670nano-PGK-Blast | Tet-inducible expression of Cas9-T2A-miRFP670nano |
| 196723 | pRDA52_Lenti.puro.U6_modified DR | For cloning pairs of AsCas12a guides with two DRs that are as different as possible. |
| 196724 | pRDA174_EF1a_EnAsCas12a_BFP | Sortable and selectable EnAsCas12a. |
| 196725 | pCMV-BFP-U6-sgRNA | Sortable sgRNA expression, Chen tracrRNA PMID: 24360272 |
| 196726 | lenti-RfxCas13d-Blast-BFP | Sortable and selectable RfxCas13d. |
| 196727 | lentiCRISPR.v2.TetON_Cas13d_U6_DR | Sortable and selectable, Tet-inducible RfxCas13d with guide cassette (one vector system). Inducible knock-down. |