Skip to main content
Addgene

APP and Bace1: Differential effect of cholesterol enrichment on processing and plasma membrane mobility.

Capitini C, Bigi A, Parenti N, Emanuele M, Bianchi N, Cascella R, Cecchi C, Maggi L, Annunziato F, Pavone FS, Calamai M
iScience. 2023 Apr 11;26(5):106611. doi: 10.1016/j.isci.2023.106611. eCollection 2023 May 19. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
196694mBFP-APP-mGFPExpresses APP695 with two fluorescent proteins at the ends to follow processing in mammalian cells
196695mBFP-APPP1-mGFPAs mBFP-APP-mGFP, but with substitution at position 612 that prevents alpha-secretase activity
196696HA-APP-mGFPExpresses APP695 with an HA tag an N-terminal for single molecule tracking and other measusers in mammalian cells
196697HA-BACE1-mBFPExpresses Bace1 with an HA tag at N-terminal for single molecule tracking and other measusers in mammalian cells
196704mCherry-APP-mGFPExpresses APP695 with two fluorescent proteins at the ends to follow processing in mammalian cells
196705mCherry-APPP1-mGFPAs mCherry-APP-mGFP, but with substitution at position 612 that prevents alpha-secretase activity
196706AP-APPExpresses APP695 with a biotin Acceptor Peptide tag at N-terminal for single molecule tracking and other measusers in mammalian cells

Antibodies from Article