APP and Bace1: Differential effect of cholesterol enrichment on processing and plasma membrane mobility.
Capitini C, Bigi A, Parenti N, Emanuele M, Bianchi N, Cascella R, Cecchi C, Maggi L, Annunziato F, Pavone FS, Calamai M
iScience. 2023 Apr 11;26(5):106611. doi: 10.1016/j.isci.2023.106611. eCollection 2023 May 19.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
196694 | mBFP-APP-mGFP | Expresses APP695 with two fluorescent proteins at the ends to follow processing in mammalian cells |
196695 | mBFP-APPP1-mGFP | As mBFP-APP-mGFP, but with substitution at position 612 that prevents alpha-secretase activity |
196696 | HA-APP-mGFP | Expresses APP695 with an HA tag an N-terminal for single molecule tracking and other measusers in mammalian cells |
196697 | HA-BACE1-mBFP | Expresses Bace1 with an HA tag at N-terminal for single molecule tracking and other measusers in mammalian cells |
196704 | mCherry-APP-mGFP | Expresses APP695 with two fluorescent proteins at the ends to follow processing in mammalian cells |
196705 | mCherry-APPP1-mGFP | As mCherry-APP-mGFP, but with substitution at position 612 that prevents alpha-secretase activity |
196706 | AP-APP | Expresses APP695 with a biotin Acceptor Peptide tag at N-terminal for single molecule tracking and other measusers in mammalian cells |