A fluorogenic chemically induced dimerization technology for controlling, imaging and sensing protein proximity.
Bottone S, Joliot O, Cakil ZV, El Hajji L, Rakotoarison LM, Boncompain G, Perez F, Gautier A
Nat Methods. 2023 Aug 28. doi: 10.1038/s41592-023-01988-8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 196693 | pAG1159 TOM20-ECFP-FIREmate | Expresses TOM20-ECFP-FIREmate in mammalian cells |
| 205164 | pAG1160 mCherry-FIREtag | Expresses mCherry -FIREtag in mammalian cells |
| 205165 | pAG1344 EGFP-FIREtag | Expresses EGFP-FIREtag in mammalian cells |
| 205166 | pAG1171 FIREmate-ECFP-Giantin | Expresses FIREmate-ECFP-Giantin in mammalian cells |
| 205167 | pAG1169 FIREmate-ECFP-Cb5 | Expresses FIREmate-ECFP-Cb5 in mammalian cells |
| 205168 | pAG1186 Lyn11-ECFP-FIREmate | Expresses Lyn11-ECFP-FIREmate in mammalian cells |
| 205169 | pAG1243 NES-ECFP-FIREmate | Expresses NES-ECFP-FIREmate in mammalian cells |
| 205170 | pAG1242 NLS-mCherry-FIREtag | Expresses NLS-mCherry-FIREtag in mammalian cells |
| 205171 | pAG1247 H2B-ECFP-FIREmate | Expresses H2B-ECFP-FIREmate in mammalian cells |
| 205172 | pAG1280 NLS-mCherry-FIREtag-NES | expresses NLS-mCherry-FIREtag-NES in mammalian cells |
| 205173 | pAG1237 FIREmate-ECFP-Fis1 | Expresses FIREmate-ECFP-Fis1 in mammalian cells |
| 205174 | pAG1238 PINK1-mCherry-FIREtag | Expresses PINK1-mCherry-FIREtag in mammalian cells |
| 205175 | pAG1244 p65-mCherry-FIREtag | Expresses p65-mCherry-FIREtag in mammalian cells |
| 205176 | pAG1345 mCherry-FIREtag-DEVDG-MoA | Expresses mCherry-FIREtag-DEVDG-MoA in mammalian cells |
| 205177 | pAG1188 mCherry-cagedFIREtag | Expresses mCherry-cagedFIREtag in mammalian cells |
| 216694 | pFP5290 ss-FIREmate-HA- KDEL-IVS-IRES- ss-SBP-mApple- FIREtag-GPI | Synchronize trafficking of GPI-AP from the ER (RUCH system) |
| 216695 | pFP5299 ss-FIREmate-HA- KDEL-IVS-IRES- ManII-SBP- mApple-FIREtag | Synchronize anterograde and retrograde trafficking of ManII between ER and Golgi (RUCH system) |
| 216696 | pFP5370 ss-FIREmate-HA- KDEL-IVS-IRES- TNF-SBP- mCherry-FIREtag | Synchronize trafficking of TNFalpha from the ER (RUCH system) |
| 216724 | FP5369 KIF17MD-flag- FIREmate | Expresses motor domain of KIF17 fused to FIREmate in mamallian cells |
| 216725 | pFP5380 LAMP1-SBP- mCherry-FIREtag | Expresses LAMP1-mCherry-FIREtag in mamallian cells |