Locomotion activates PKA through dopamine and adenosine in striatal neurons.
Ma L, Day-Cooney J, Benavides OJ, Muniak MA, Qin M, Ding JB, Mao T, Zhong H
Nature. 2022 Nov;611(7937):762-768. doi: 10.1038/s41586-022-05407-4. Epub 2022 Nov 9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 196675 | pAAV_SynP_DIO_tAKARα _WPRE | AAV plasmid for mammalian Cre-dependent expression of tAKARα (genetically encoded sensor for protein kinase A). |
| 196676 | pAAV_SynP_DIO_tAKARα- T391A_WPRE | AAV plasmid for mammalian Cre-dependent expression of the T391A mutant of tAKARα (abolished sensor phosphorylation site). |
| 196677 | pAAV_CAG_FLEX_PKIα-P2A-mRuby3_WPRE | AAV plasmid for mammalian Cre-dependent expression of PKIα-P2A-mRuby3 under a CAG promotor w/ WPRE enhancer. PKIα (protein kinase inhibitor α) inhibits the activity of protein kinase A (PKA). |
| 196678 | pAAV_CAG_FLEX_PKIαmut-P2A-mRuby3_WPRE | AAV plasmid for mammalian Cre-dependent expression of PKIαmut-P2A-mRuby3 under a CAG promotor w/ WPRE enhancer. PKIαmut bears the R20G/R21G mutation that renders it non-functional. |