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The lipid linked oligosaccharide polymerase Wzy and its regulating co-polymerase, Wzz, from enterobacterial common antigen biosynthesis form a complex.

Weckener M, Woodward LS, Clarke BR, Liu H, Ward PN, Le Bas A, Bhella D, Whitfield C, Naismith JH
Open Biol. 2023 Mar;13(3):220373. doi: 10.1098/rsob.220373. Epub 2023 Mar 22. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
196661pHis-WzzEExpression of N-terminally His6-tagged WzzE from Pectobacterium atrosepticum in E.coli
196662pGST-WzzEExpression of N-terminally GST-tagged WzzE from Pectobacterium atrosepticum in E.coli
196663pHis-mCherry-WzzEExpression of N-terminally His6-mCherry-tagged WzzE from Pectobacterium atrosepticum in E.coli
196664pPglL-strep-mCherryExpression of C-terminally distrep tag II-mCherry-tagged PglL from Neisseria meningitidis in E.coli
196665pPglL-strep-mClover3Expression of C-terminally distrep tag II-mClover3-tagged PglL from Neisseria meningitidis in E.coli
196666pWzyE-GST-distrepIIExpression of C-terminally GST-distrep tag II-tagged WzyE from Pectobacterium atrosepticum in E.coli
196667pWzyE-distrepIIExpression of C-terminally distrep tag II-tagged WzyE from Pectobacterium atrosepticum in E.coli
196668pWzyE-strep-mClover3Expression of C-terminally distrep tag II-mClover3-tagged WzyE from Pectobacterium atrosepticum in E.coli
196669pWzyE-HisExpression of C-terminally His10-tagged WzyE from Pectobacterium atrosepticum in E.coli

Antibodies from Article