Ultradeep characterisation of translational sequence determinants refutes rare-codon hypothesis and unveils quadruplet base pairing of initiator tRNA and transcript.
Hollerer S, Jeschek M
Nucleic Acids Res. 2023 Mar 21;51(5):2377-2396. doi: 10.1093/nar/gkad040.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 196656 | pASPIre4 | Derivative of pASPIre3. Contains SpeI restriction site within the CDS of bxb1 to enable diversification of the 5’-UTR and codons 2-16. |
| 196657 | ptRNAfMet-A37 | Plasmid for overexpression of native initiator tRNA (A37) |
| 196658 | ptRNAfMet-A37G | Plasmid for overexpression of mutated initiator tRNA (A37G) |
| 196659 | ptRNAfMet-A37U | Plasmid for overexpression of mutated initiator tRNA (A37U) |