Transient inhibition of p53 enhances prime editing and cytosine base-editing efficiencies in human pluripotent stem cells.
Li M, Zhong A, Wu Y, Sidharta M, Beaury M, Zhao X, Studer L, Zhou T
Nat Commun. 2022 Oct 27;13(1):6354. doi: 10.1038/s41467-022-34045-7.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 196582 | pCMV-PE2-p53DD | Mammalian expression of SpCas9 prime editor 2 with P2A-p53DD |
| 199267 | pEF_PE2 | Mammalian expression of SpCas9 prime editor 2 under EF1α promoter |
| 199271 | pegRNA-GBA-10 | pegRNA used to induce GBA (c. 1226 A > G) mutation |
| 199272 | pegRNA_LRRK2-3 | pegRNA used to induce LRRK2 (c. 6055 G > A) mutation |
| 199273 | pegRNA_LMNA-1 | pegRNA used to induce LMNA (c.1824C > T) mutation |
| 199274 | pegRNA_GBA-4 | pegRNA used to correct GBA (c. 1226 A > G) mutation |
| 199275 | pegRNA_LMNA_CR-2-5 | pegRNA used to correct LMNA (c.1824C > T) mutation |
| 199276 | Lsg-GBA-1 + 2 | nicking sgRNA to induce or correct GBA (c. 1226 A > G) mutation using PE3 |
| 199277 | Lsg-LRRK2-1 | nicking sgRNA to induce LRRK2 (c. 6055 G > A) mutation using PE3 |
| 199278 | Lsg-LMNA-1 + 2 | nicking sgRNA to induce LMNA (c.1824C > T) mutation using PE3 |