Site-specific incorporation of unnatural amino acids into proteins in mammalian cells.
Hino N, Sakamoto K, Yokoyama S
Methods Mol Biol. 2012;794:215-28. doi: 10.1007/978-1-61779-331-8_13.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 196483 | pcpAzpaRS_v2 | To express azidophenylalanine-specific variant of E. coli TyrRS (pAzpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into azidophenylalanine. |
| 196484 | pcpBpaRS | To express benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into benzoylphenylalanine. |
| 196485 | pcpBpaRS_v2 | To express benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into benzoylphenylalanine. |
| 196486 | pcPylRS(Y384F Y306A) | To express Methanosarcina mazei PylRS(Y384F Y306A) and allow UAG codon to be translated into Z-Lys or Azido-Z-Lys when used together with the pU6tRNAPyl plasmid. |