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GEARBOCS: An Adeno Associated Virus Tool for In Vivo Gene Editing in Astrocytes.

Bindu DS, Tan CX, Savage JT, Eroglu C
bioRxiv. 2023 Jan 19:2023.01.17.524433. doi: 10.1101/2023.01.17.524433. Preprint. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
196488pgfaABC1D-mCherry-CAAXTo over express mCherry-CAAX under gfaABC1D promoter in astrocytes
196489pGEARBOCS-v0-Gfap-KOTo KO Gfap in mouse astrocytes
196490pGEARBOCS-v0- Gfap-TagIN-mCherryTo tag endogenous mouse GFAP with mCherry
196491pGEARBOCS-v0-Gfap- TagIN-HATo tag endogenous mouse GFAP with HA
196492pGEARBOCS-v0-Gfap-GeneTRAPTo KO and genetrap mouse GFAP simultaneously
196493pGEARBOCS-v0-Vamp2-GeneTRAPTo KO and genetrap mouse VAMP2 simultaneously
196494pGEARBOCS-v0-Vamp2-TagIN-HATo tag endogenous mouse VAMP2 with HA
196495pGEARBOCS-v0CRISPR tool to target astrocytes
218181GEARBOCSCRISPR tool to target astrocytes
218182GEARBOCS-SPARCL1 KOTo KO Hevin in astrocytes
218183GEARBOCS-SPARCL1-C-mCherryTagTo tag Hevin with mCherry at its C-terminal
218184GEARBOCS-SPARCL1-GeneTRAPTo KO and genetrap mouse Hevin simultaneously
218185GEARBOCS-SPARCL1-N-HATagTo tag Hevin with HA at the N-terminal
218186GEARBOCS-SPARCL1-N-mCherryTagTo tag Hevin with mCherry at the N-terminal
218187GEARBOCS-Vamp2-GeneTRAPTo KO and genetrap mouse Vamp2 simultaneously
218188GEARBOCS-Vamp2-Tag-HATo tag endogenous mouse VAMP2 with HA
218189pAAV-gfaABC1D-mCherry-CAAXTo over express mCherry-CAAX under gfaABC1D promoter in astrocytes

Antibodies from Article