B(12)-induced reassembly of split photoreceptor protein enables photoresponsive hydrogels with tunable mechanics.
Yang Z, Fok HKF, Luo J, Yang Y, Wang R, Huang X, Sun F
Sci Adv. 2022 Apr;8(13):eabm5482. doi: 10.1126/sciadv.abm5482. Epub 2022 Apr 1.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 196474 | p32m3C Trx-CN | Protein expression of Thiolredoxin-3C cleavage site-CarHCN |
| 196475 | p32m3C Trx-CC | Protein expression of Thiolredoxin-3C cleavage site-CarHCC |
| 196477 | pQE80l B-CN-B | Protein expression of SpyCatcher-ELP-CarHCN-ELP-SpyCatcher, with TEV cleavage site and MMP cleavage site respectively before and after each SpyCatcher, and GRGD site linked with (GGGGS)2 before CarHCN |
| 196478 | pQE80l A-CC-A | Protein expression of SpyTag-ELP-CarHCC-ELP SpyTag, with GRGD site linked with (GGGGS)2 before CarHCC and after first ELP |