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Avidity-based bright and photostable light-up aptamers for single-molecule mRNA imaging.

Buhler B, Schokolowski J, Benderoth A, Englert D, Grun F, Jaschke A, Sunbul M
Nat Chem Biol. 2023 Apr;19(4):478-487. doi: 10.1038/s41589-022-01228-8. Epub 2023 Jan 19. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
196344pAV-U6+27-Tornado-biRhoBASTOverexpression of circular biRhoBAST
196345pAV-U6+27-Tornado-biSiRAOverexpression of circular biSiRA
196348mEGFP-biRhoBASTExpression of mEGFP mRNA with biRhoBAST in 3'UTR
196349mEGFP-2xbiRhoBASTExpression of mEGFP mRNA with 2xbiRhoBAST in 3'UTR
196350mEGFP-4xbiRhoBASTExpression of mEGFP mRNA with 4xbiRhoBAST in 3'UTR
196351mEGFP-8xbiRhoBASTExpression of mEGFP mRNA with 8xbiRhoBAST in 3'UTR
196352mAzurite-8xbiRhoBAST-24xMS2v6Expression of mAzurite mRNA with 8xbiRhoBAST-24xMS2v6 in 3'UTR

Antibodies from Article