Engineered biocontainable RNA virus vectors for non-transgenic genome editing across crop species and genotypes.
Liu Q, Zhao C, Sun K, Deng Y, Li Z
Mol Plant. 2023 Mar 6;16(3):616-631. doi: 10.1016/j.molp.2023.02.003. Epub 2023 Feb 7.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 196286 | pL | 35S promoter-driven expression of the positive-sense TSWV L RNA segment encoding codon-optimized L (RdRp) |
| 196291 | pM-Cas12a | 35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding LbCas12a in place of viral GP |
| 196292 | pM-ABE | 35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding ABE in place of viral GP |
| 196293 | pM-CBE | 35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding CBE in place of viral GP |
| 196295 | pS-cr:GFP | 35S promoter-driven expression of the positive-sense TSWV S RNA segment encoding crRNA:GFP fusion in place of the NSs |
| 196296 | pS-sg:GFP | 35S promoter-driven expression of the positive-sense TSWV S RNA segment encoding sgRNA:GFP fusion in place of viral NSs |
| 196325 | pM-Cas9 | 35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding SpCas9 in place of viral GP |