A dual sgRNA library design to probe genetic modifiers using genome-wide CRISPRi screens.
Guna A, Page KR, Replogle JM, Esantsi TK, Wang ML, Weissman JS, Voorhees RM
BMC Genomics. 2023 Oct 30;24(1):651. doi: 10.1186/s12864-023-09754-y.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 196280 | pJR152 | Cassette used in dual sgRNA library generation |
| 197348 | mU6-CR1-CRISPRiv2_hU6-CR3-NT-ctrl-guide_EF1a-PuroR-T2A-BFP | To allow for genome-wide genetic modifier screening, the library has a second fixed non-targeting control guide. |
| 197349 | mU6-CR1-CRISPRiv2_hU6-CR3-EMC2-guide_EF1a-PuroR-T2A-BFP | To allow for genome-wide genetic modifier screening, the library has a second fixed guide targeting EMC2. |