Intron-encoded cistronic transcripts for minimally invasive monitoring of coding and non-coding RNAs.
Truong DJ, Armbrust N, Geilenkeuser J, Lederer EM, Santl TH, Beyer M, Ittermann S, Steinmassl E, Dyka M, Raffl G, Phlairaharn T, Greisle T, Zivanic M, Grosch M, Drukker M, Westmeyer GG
Nat Cell Biol. 2022 Nov;24(11):1666-1676. doi: 10.1038/s41556-022-00998-6. Epub 2022 Nov 7.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 197263 | pINSPECT_SigP:NLuc | Cloning Backbone for INSPECT expression. Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via Esp3I. |
| 197264 | pINSPECT_5'-xrRNA_NLuc_3'-XAP1 | Cloning Backbone for enhanced INSPECT expression (5' xrRNA + 3' XAP1). Encodes for intronic IRES-driven NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via BsaI. |
| 197265 | pINSPECT_5'-xrRNA_NLuc_3'-HCV-UTR | Cloning Backbone for enhanced INSPECT expression (5' xrRNA + 3' HCV-UTR). Encodes for intronic IRES-driven NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via BsaI. |
| 197266 | pINSPECT:IL2_SigP:NLuc | Donor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human IL2 locus (exon 3). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. |
| 197267 | pINSPECT:NEAT1-total_SigP:NLuc | Donor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human NEAT1 locus (both isoforms). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. |
| 197268 | pINSPECT:NEAT1-v2_SigP:NLuc | Donor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human NEAT1 locus (long isoform only). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. |