Efficient CRISPR-Cas9 based cytosine base editors for phytopathogenic bacteria.

Li C, Wang L, Cseke LJ, Vasconcelos F, Huguet-Tapia JC, Gassmann W, Pauwels L, White FF, Dong H, Yang B
Commun Biol. 2023 Jan 17;6(1):56. doi: 10.1038/s42003-023-04451-8. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
196247	pHMattR3-attR4-GFP	pHM1 derived plasmid containing the Gateway cassette attR3-attR4 and GFP gene
196248	pEN-dCas9-CDA1-UGI	Plasmid containing promoter of RecA from Xanthomonas oryzae driving expression of dead Cas9, cytosine deaminase CDA1, and glycosylase inhibitor
196249	pEN-sacB	Plasmid containing the sacB gene
196250	pEN-L1-PJ23119-PplpT-sfGFP-TrrfB-BsaI-Scaf-L2	Plasmid for cloning of a CRISPR-Cas9 guide RNA gene under promoter PJ23119
196251	pTL-Bag1-gRNA1	Plasmid for cloning the first CRISPR-Cas9 guide RNA of multiplex guide RNAs
196252	pTL-Bag2-gRNA2	Plasmid for cloning the second CRISPR-Cas9 guide RNA of multiplex guide RNAs
196253	pTL-Bag2T-gRNA2	Plasmid for cloning the second CRISPR-Cas9 guide RNA for guide RNAs
196254	pTL-Bag3-gRNA3	Plasmid for cloning the third CRISPR-Cas9 guide RNA of 4 guide RNAs
196255	pTL-Bag4-gRNA4	Plasmid for cloning the 4th CRISPR-Cas9 guide RNA of 4 guide RNAs