CRISPR-Cas-Guided Mutagenesis of Chromosome and Virulence Plasmid in Shigella flexneri by Cytosine Base Editing.

Sharma A, Omer Aden R, Puhar A, Cisneros DA
mSystems. 2022 Dec 21:e0104522. doi: 10.1128/msystems.01045-22. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
196242	pnCas9-AID	Plasmid expressing the nCas9-AID fusion
196243	pgRNA_AT	Backbone plasmid for cloning sgRNA
220927	pgRNA-R-gfp	Expresses gfp using J23119 promoter and pgRNA_AT backbone