Peptide-mediated delivery of CRISPR enzymes for the efficient editing of primary human lymphocytes.
Foss DV, Muldoon JJ, Nguyen DN, Carr D, Sahu SU, Hunsinger JM, Wyman SK, Krishnappa N, Mendonsa R, Schanzer EV, Shy BR, Vykunta VS, Allain V, Li Z, Marson A, Eyquem J, Wilson RC
Nat Biomed Eng. 2023 May;7(5):647-660. doi: 10.1038/s41551-023-01032-2. Epub 2023 Apr 25.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 196244 | pRM053 His-TEV-Cas9 triNLS 0C | Expression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized) |
| 196245 | pRM049 His-TEV-Cas9 triNLS 2C | Expression of NLS-rich Cas9 (2 Cys residues, mammalian codon optimized) |
| 196246 | pRM055 His-TEV-Cas9 6xNLS 0C | Expression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized) |